目的 研究细梗香草皂苷B(capilliposide B,LC-B)和细梗香草皂苷C(capilliposide C,LC-C)在不同温度,pH条件下水解动力学,及水解产物的鉴定。方法 采用高效液相色谱法测定LC-B,LC-C含量,推导出LC-B,LC-C水解过程,恒温30,50和70 ℃,pH分别为9.0,10.0,11.0 的条件下,不同时间点分别取样检测LC-C和LC-B的含量,探讨其稳定性,并通过质谱、核磁共振谱鉴定水解产物。结果 LC-B,LC-C在不同温度,pH条件下的水解反应均符合一级动力学。水解速率受水解的温度和体系pH值的影响:相同的水解温度,pH越高,水解越快;相同pH,温度越高水解越迅速。水解产物为细梗香草皂苷A(capilliposide A,LC-A)。 结论 该实验为LC-A的制备提供一种有效方法。
Abstract
OBJECTIVE To explore the hydrolysis kinetics of capilliposide B and capilliposide C under different temperatures and pH values, and identify the hydrolysis products. METHODS The LC-B and LC-C were quantified by HPLC, then the hydrolysis process was deduced. Samples were taken to investigate the stability of LC-C and LC-B at different time points under the conditions of 30, 50, 70 ℃, and pH 8.0, 9.0, 10.0, 11.0, respectively. The hydrolysis products were identified by mass spectrometry and nuclear magnetic resonance spectroscopy. RESULTS The hydrolysis reaction rates of LC-B and LC-C obeyed first-order kinetics under different pH values and temperatures. And they were related to hydrolysis temperature and pH: at the same temperature, the higher the pH, the faster the hydrolysis rate; at the same pH, the higher the temperature, the faster the hydrolysis rate. The hydrolysis product was capilliposide A. CONCLUSION The study provided an effective method for the preparation of LC-A.
关键词
细梗香草皂苷A /
细梗香草皂苷B /
细梗香草皂苷C /
水解动力学
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Key words
capilliposide A /
capilliposide B /
capilliposide C /
hydrolysis kinetics
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金资助项目(81603078);江苏省科技支撑项目资助(BE2014654);常熟市科技支撑项目资助(CS201405)
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